Biological impact, oxidative stress and adipokinetic hormone activities of Agrotis ipsilon in response to bioinsecticides

The use of biological control products enhances the reduction harmful pressures on environment caused by conventional pesticides. Therefore, this study aims to evaluate toxicity eight bioinsecticides black cutworm Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) understand relationships between insecticide exposure and insect response at individual population levels. bioassay results showed that emamectin benzoate had high against A. second instar larvae with an LC50 0.007 (mg/L). Sublethal concentration (LC30) benzoate, spinosyn group Bacillus thuringiensis formulation (Dipel 2X) significantly prolonged pupal period. In addition, decreased number eggs laid per female (135.3 ± 6.919) compared treatment. Thus, chlorfenapyr a 1.26-fold in hatching rate. Meanwhile, activities oxidative stress enzymes (catalase, superoxide dismutase, lipid peroxidase, glutathione reductase) were affected due sublethal concentration. Changes adipokinetic hormone (AKH) transcriptional regulation detected via SQ-PCR using cDNA synthesized from mRNA isolated treated larvae. higher transcription rate AKH spinotram-treated 1.42-fold increase over untreated Our provide useful information for integrated pest management programs bioinsecticides.